https://www.oliverstringham.com/tags/environmental-dna/ Recent content in environmental dna on Oliver C. Stringham Hugo -- gohugo.io en Mon, 01 Jul 2024 00:00:00 +0000 https://www.oliverstringham.com/publications/edna-khapra-beetle-stored-rice/ Mon, 01 Jul 2024 00:00:00 +0000 https://www.oliverstringham.com/publications/edna-khapra-beetle-stored-rice/ We developed an eDNA method — washing and filtering stored rice, then screening with qPCR — to detect khapra beetle, one of the world’s most damaging stored-product pests. The method detected a single larva within a 50 kg container of rice with 85–97%+ certainty, depending on the number of qPCR replicates run. https://www.oliverstringham.com/publications/surface-edna-arthropod-biodiversity-forest/ Mon, 06 Nov 2023 00:00:00 +0000 https://www.oliverstringham.com/publications/surface-edna-arthropod-biodiversity-forest/ We collected environmental DNA from tree bark and understory foliage at 40 sites in a New Jersey forest and, using two metabarcoding primer sets, recovered over 1,000 arthropod molecular operational taxonomic units spanning 292 identified species, 181 families, and 28 orders — proof-of-concept that surface eDNA can survey terrestrial arthropod communities.